196.
Sandoval, G., Andrews, B.
and Asenjo, J.A. (2012). Elution relationships to model affinity chromatography
using a general rate model. J.of Molecular
Recognition, in
press.
195.
Moisset, P., Vaisman, D.,
Cintolesi, A., Urrutia, J., Rapaport, I., Andrews, B.A. and Asenjo, J.A. (2012)
Continuous Modeling of Metabolic Networks with Gene Regulation in Yeast and In
Vivo Determination of Rate Parameters. Biotechnology & Bioengineering, Vol.
109, No. 9, 2325-2339.
194.
Santhanam, R., Okoro,
Ch.K., Rong, X., Huang, Y., Bull, A.T., Weon, H-Y, Andrews, B.A., Asenjo, J.A.
& Goodfellow, M. (2012) Streptomyces atacamensis sp. nov., isolated from an
extreme hyper-arid soil of the Atacama Desert. International Journal of
Systematic and Evolutionary Microbiology, doi:10.1099/ijs.0.038463-
193.
Mayolo-Deloisa, K., Lienqueo,
ME, Andrews, B.A., Rito-Palomares, M. and Asenjo, J.A. (2012) Hydrophobic
Interaction Chromatography for Purification of MonoPEGylated RNase A. Journal
of Chromatography A. 1242, 11-16.
192.
Santhanam, R ., Okoro,
Ch.K., Rong, X., Huang, Y., Bull, A.T., Andrews, B.A., Asenjo, J.A., Weon, H-Y. &
Goodfellow, M. (2012) Streptomyces
deserti sp. Nov., isolated from hyper-arid Atacama Desert soil. Antonie van
Leeuwenhoek, 101, 575-581.
191.
Asenjo, J.A. and Andrews,
B.A. (2012) Aqueous two-phase systems
for protein separation: Phase separation and applications. Journal of
Chromatography A. 1238, 1-10.
190.
Nachtigall, J., Kulik,
A., Helaly, S., Bull, A., Goodfellow, M., Asenjo, J.A., Maier, M., Wiese, J.,
Imhoff, J.F., Süssmuth, R. D. & Fiedler, H-P. (2011) Atacamycins A-C,
22-membered antitumor macrolactones produced by Streptomyces sp C38*, The
Journal of Antibiotics, 64, 775-780.
189.
Benavides, J.,
Rito-Palomares, M. and Asenjo J.A. (2011) Aqueous Two-Phase Systems.
Comprehensive Biotechnology. Ed. C. Webb, Elsevier L.V., Vol. 2, 697-713,
doi:10.1016/B978-0-08-088504-9 00124-0.
188.
Rateb, M.E., Houssen,
W.E., Harrison, W.T.A., Deng, H., Okoro, Ch.K., Asenjo, J.A., Andrews, B.A.,
Bull, A.T., Goodfellow, M., Rainer, E. and Jaspars, M. (2011) Diverse Metabolic
Profiles of a Streptomyces Strain Isolated from a Hyper-arid Environment.
Journal of Natural Products, 74,
1965-1971.
187.
Contador, C., Andrews,
B., Liao, J., and Asenjo J.A. (2011) Identification of Transcription Factors
perturbed by the Synthesis of High Levels of a Foreign Protein in Yeast Saccharomyces cerevisiae. Biotechnology Progress, 27,
925-936.
186.
Rateb, M.E., Houssen,
W.E., Arnold, M., Abdelrahman, M.H., Deng, H., Harrison, W.T.A., Okoro, Ch.K.,
Asenjo, J.A., Andrews, B.A., Ferguson, G., Bull, A.T., Goodfellow, M., Ebel, R.
and Jaspars, M. (2011) Chaxamycins A-D,
Bioactive Ansamycins from a Hyper-arid Desert Streptomyces sp. Journal of
Natural Products, 74, 1491-1499.
185.
Asenjo, J. A. and
Andrews, B. A. (2011) Aqueous Two-Phase Systems for Protein Separation. Journal
of Chromatography A., 1218, 8826-8835.
184.
Olivera, A., Andrews,
B.A. and Asenjo, J.A. (2011) Mutagenesis Objective Search and Selection Tool
(MOSST): an algorithm to predict structure-function related mutations in
proteins. BMC Bioinformatics, 12,
122, 1-22.
183.
Lienqueo, ME., Shene, C.,
Quiroga, A., Salazar, O., Salgado, J.C. and Asenjo, J.A. (2010) Experimental
Validation of the Predictions of a Mathematical Model for Protein Purification
and Tag Selection. Separation Science and Technology, 45, 2153-2164.
182.
Tan Y., Rivera L.,
Contador C., Asenjo J.A., Liao J. (2010) Reducing the Allowable Kinetic Space
by Constructing Ensemble of Dynamic Models with the Same Steady-State Flux.
Metabolic Engineering, 13, 60-75.
181.
Sandoval, G., Shene, C.,
Andrews, B.A. and Asenjo, J.A. (2010) Extension of the Selection of Protein
Chromatography and the Rate Model to Affinity Chromatography. Journal of
Molecular Recognition, 23, 609-617.
180.
Merino, M.P. Andrews, B.A. and Asenjo, J.A. (2010)
Stoichiometric Model and Metabolic Flux Analysis for Leptospirilum
ferrooxidans. Biotechnology and Bioengineering, 107, 696-706.
179.
Sepúlveda, D.E., Andrews,
B.A., Papoutsakis, E.T. and Asenjo, J.A. (2010) Metabolic Flux Analysis of
Embryonic Stem Cells Using Three Distinct Differentiation Protocols and
Comparison to Gene Expression Patterns. Biotechnology Progress, 26, 1222-1229.
178.
Andrews, B.A. and Asenjo,
J.A. (2010) Theoretical and Experimental Evaluation of Hydrophobicity of
Proteins to Predict their Partitioning Behaviour in Aqueous Two Phase Systems:
a Review. Separation Science and Technology. 45, 2165-2170.
177.
Olivera, A., Picioreanu, C.,
Asenjo, J.A. (2010) Non-Homogeneous Biolfim Modeling Applied to Bioleaching
Processes. Biotechnology and Bioengineering, 106, 660-676.
176.
Martinez, V., Gerdtzen,
Z.P., Andrews, B.A. and Asenjo, J.A. (2010) Viral Vectors for the Treatment of
Alcoholism: use of Metabolic Flux Analysis for Cell Cultivation and Vector
Production. Metabolic Engineering, 12,
129-137.
175.
Lienqueo, M.E., Shene, C.
and Asenjo, J.A. (2009) Optimization of hydrophobic interaction chromatography
using a mathematical model of elution curves of a protein mixture. J. Molec.
Recognit. 22, 110-120.
174.
Contador, Carolina A.,
Rizk, Matthew L., Asenjo, J.A. and Liao, James C. (2009) Ensemble modeling for
strain development of L-lysine-producing
Escherichia coli. Metabolic Engineering, 11, 221-233.
173.
Díaz, H., Andrews, B.A.,
Hayes, A., Castrillo, J., Oliver, S. and Asenjo, J.A. (2009) Global Gene Expresion in Recombinant and Non-Recombinant
Yeast Saccharomyces cerevisiae In Three Different Metabolic States. Biotechnology
Advances. 27, 1092-1117.
172.
Orellana, C.A., Shene, C.
and Asenjo, J.A. (2009) Mathematical Modelling of Elution Curves for a Protein
Mixture in Ion Exchange Chromatography Applied to High Protein Concentration.
Biotechnology and Bioengineering, 104,
572-581.
171.
Gerdtzen, Z.P., Salgado,
J.C., Osses, A., Asenjo, J.A., Rapaport, I. and Andrews, B. (2009) Modeling
heterocyst pattern formation in cyanobacteria. Bio Med Central Bioinformatics. 10 (Suppl 6):S16 .
170.
Lienqueo, M.E., Salgado,
J.C., Giaverini, O., Asenjo, J.A. (2009) Computer-aided design to select
optimal polypeptide tags to assist the purification of recombinant proteins.
Separation and Purification Technology, 65 86-94.
169.
Okoro, C.K., Brown, R, Jones, A.
L., Andrews, B., Asenjo, J.A., Goodfellow, M. and Bull, A. T. (2009) Actinomycete
diversity in hyper-arid soils of the Atacama Desert, Chile. Antonie van
Leeuwenhoek International Journal of General and Molecular Microbiology, 95, 121-133.
168.
Hold, C., Andrews, B. and Asenjo, J.A. (2009) A Stoichiometric
Model of Acidithiobacillus ferrooxidans
ATTC 23270 for Metabolic Flux Analysis. Biotechnology and Bioengineering, 102, 1448-1459.
167.
Asenjo, J.A. and Andrews, B.A. (2009) Protein Purification using
Chromatography: Selection of Type, Modelling, and Optimization of Operating
Conditions. Journal of Molecular Recognition. 22, 65-76.
166.
Salgado, J.A, Andrews
B.A., Ortuzar, M.F. and Asenjo, J.A. (2008) Prediction of the partitioning
behaviour of proteins in aqueous two-phase systems using only their amino acid
composition. Journal of Chromatography
A, 1178,
134-144.
165.
Sepulveda, D.E., Andrews,
B.A., Asenjo, J.A. and Papoutsakis, E.T. (2008) Comparative Transcriptional
Analisys of Emryoid Body versus Two-Dimensional Differentiation of Murine
Embryonic Stem Cells. Tissue Engineering. Part A, 14, 1603-1614.
164.
Acevedo, J.P., Reyes F.,
Parra, L., Salazar, O., Andrews, B.A. and Asenjo, J.A. (2008) Cloning of
complete genes for novel hydrolytic enzymes from Antarctic sea water bacteria
by use of an improved genome walking technique. Journal of Biotechnology, 133,
277-286.
163.
Asenjo, J.A. and Andrews,
B.A. (2008) Challenges and Trends in Bioseparations, J. Chem Tech. Biotecnol., 83,117–120.
162.
Parra L., Reyes F., Acevedo J.P., Salazar O., Andrews B., Asenjo J.,
(2008) Cloning and fusion expression of a cold-active lipase from marine
Antarctic origin. Enzyme and Microbial Technology, 42, 371-377.
161.
Kaltenbrunner O., Giaverini O., Woehle D. and Asenjo J.A. (2007)
Application of Chromatographic Theory for Process Characterization Towards
Validation of an lon-Exchange Operation. Biotechnol.Bioeng., 98, 201-210.
160.
Salazar O. and Asenjo J.A. (2007), Enzymatic lysis of microbial cells.
Biotechnol Lett, 29, 985 – 994.
159.
Asenjo, J.A., Ramirez,
P., Rapaport, I., Aracena, J., Goles, E., and Andrews, B.A. (2007) A Discrete Mathematical Model Applied to
Genetic Regulation and Metabolic Networks. J. of Microbiology and Biotechnology 17, 496-510.
158.
Lienqueo, M.E, Mahn A.,
Salgado, J.C. and Asenjo, J.A. (2007) Current insights on protein behaviour in
hydrophobic interaction chromatography. Journal of Chromatography B. 849, 53-68.
157.
Mahn, A., Lienqueo, M.E.
and Asenjo, J.A. (2007) Optimal Operation Conditions for Protein Separation in
Hydrophobic Interaction Chromatography. J. of Chromatography B. 849, 236-242.
156.
Shene, C., Lucero, A.,
Andrews, B.A. and Asenjo, J.A (2006) Mathematical Modelling of Elution Curves
for a Protein Mixture in Ion Exchange Chromatography and for the Optimal
Selection of Operational Conditions. Biotechnol.Bioeng 95, 704-713.
155.
Lienqueo, M.E., Mahn, A.,
Navarro, G., Perez-Acle, T., Salgado, J.C., Rapaport, I., Asenjo, J.A. (2006),
New approaches for predicting protein retention time in hydrophobic interaction
chromatography. J. Molec. Recog. 19, 260-9.
154.
Salazar, O., Basso, C., Barba, P., Orellana, C., Asenjo,
J.A. (2006) Improvement of the Lytic Properties of a beta-1,3-Glucanase by
Directed Evolution. Molecular Biotechnology. 33, 211-220.
153.
Salgado, C., Rapaport,
I., and Asenjo, J.A. (2006) Predicting the behaviour of proteins in hydrophobic
interaction chromatography, 2: using a statistical description of their surface
amino acid distribution, J. Chromatography A. 1107, 120-129.
152.
Salgado, C., Rapaport, I.
and Asenjo, J.A. (2006) Predicting the behaviour of proteins in hydrophobic
interaction chromatography, 1: using the hydrophobic imbalance (HI) to describe
their surface amino acid distribution J. Chromatography A., 1107, 110-119.
151.
Ezquer, F., Núñez, M.T.,
Asenjo, J.A. and Israel, Y., (2006) Hereditary Hemochromatosis: an opportunity
for gene therapy. Biol. Res., 39, 113-124.
150.
Salgado, C., Rapaport, I.
and Asenjo, J.A (2005) Prediction of retention times of proteins in
hydrophobic interaction chromatography using only their amino acid composition.
J. of Chromatography A. 1098, 44-54.
149.
Mahn, A., Asenjo, J.A.,
(2005) Prediction of protein retention in hydrophobic interaction
chromatography. Biotechnology Advances 23,
359 - 368.
148.
Salgado, C., Rapaport, I.
and Asenjo, J.A., (2005) Is it possible to predict the average surface
hydrophobicity of a protein using only its amino acid composition? J. of
Chromatography A, 1075, 133-143.
147.
Mahn, A., Zapata, G. and
Asenjo, J.A., (2005) A theory of protein-resin interaction in hydrophobic
interaction chromatography, J. of Chromatography A, 1066, 81-88.
146.
Andrews, B.A., Schmidt,
A.S. and Asenjo, J.A., (2005) Correlation for the Partition Behaviour of
Proteins in Aqueous Two Phase Systems: Effect of Surface Hydrophobicity and
Charge. Biotechnol.Bioeng 90, 380 –
390.
145.
Olivera-Nappa, A.,
Lagomarsino, G., Andrews, B.A. and Asenjo, J.A., (2004) Effect of Electrostatic
Energy on Partitioning of Proteins in Aqueous Two-Phase Systems, J. of
Chromatography B, 807, 81-86.
144.
Mahn, A., Lienqueo, M.E.
and Asenjo, J.A. (2004) Effect of Surface Hydrophobicity Distribution on
Protein Retention in Hydrophobic Interaction Chromatography. J. of
Chromatography A, 1043, 47-55.
143.
Iribarren, O.A.,
Montagna, J.M., Vecchietti, A.R., Andrews, B.A., Asenjo J.A., and Pinto J.M.
(2004) Optimal Process Synthesis for the Production of Multiple Recombinant Proteins.
Biotechnolgy Progress 20 , 1032 – 42.
142.
Asenjo, J.A. and Andrews,
B.A. (2004) Is there a Rational Method to Purify Proteins? : From Expert
Systems to Proteomics. J.of Molecular Recognition, 17, 236-247.
141.
Olivera-Nappa, A,
Andrews, B.A. and Asenjo J.A., (2004) A mixed mechanistic-electrostatic model
to explain pH dependence of glycosyl hydrolase enzyme activity.
Biotechnol.Bioeng., 86, 573 – 586.
140.
Lienqueo, M.E., Mahn A.,
Vásquez L. and Asenjo J.A., (2003) Methodology for predicting the separation of
proteins by hydrophobic interaction chromatography and its application to a
cell extract. J. Chromatography A, 1009,
189 -196.
139.
Shene, C., Andrews B.A.
and Asenjo J.A., (2003) Study of recombinant micro-organism populations
characterized by their plasmid content per cell using a segregated model.
Bioprocess and Biosyst. Eng. 25,
333-340.
138.
Gonzalez, R., Andrews,
B.A., Molitor, J. and Asenjo, J.A. (2003) Metabolic analysis of the synthesis
of high levels of intracellular human SOD in S. Cerevisiae rhSOD 2060 411
SGA122. Biotechnol. Bioeng. 82,
152-169.
137.
Lienqueo, M.E., Mahn A.
and Asenjo J.A. (2002) Mathematical correlations for predicting protein
retention times in hydrophobic interaction chromatography. J. Chromatography A, 978, 71-79.
136.
Berggren, K., Wolf, A.,
Asenjo, J.A., Andrews, B.A. and Tjerneld, F. (2002) The surface exposed amino
acid residues of monomeric proteins determine the partitioning in aqueous
two-phase systems. Biochim. Biophys. Acta. 1596, 253-268.
135.
Salamanca, M.H., Barria,
C., Asenjo, J.A. and Andrews, B.A. (2002) Isolation, purification and
preliminary characterization of cryophilic proteases of marine origin.
Bioseparation. 10, 237 – 241.
134.
Gonzalez, R., Andrews,
B.A. and Asenjo, J.A. (2002) Kinetic model for BiP- and PDI- mediated protein
folding and assembly. J. Theor. Biol., 214,
529-537.
133.
Asenjo, J.A., Mistry,
S.L., Andrews, B.A. and Merchuk, J.C. (2002) Phase separation rates of aqueous
two-phase systems: correlation with system properties. Biotechnol. Bioeng.79, 217-223.
132.
Graber, T.A.,
Galleguillos, H., Asenjo, J.A. and Andrews, B.A. (2002) Refractive Index,
Density, and Viscosity in the NaNO3 + H2O + Poly(ethylene glycol) System at
various Temperatures. J. Chem. Eng. Data, 47,
174 – 178.
131.
Salazar, O., Molitor, J.,
Lienqueo, M.E. and Asenjo, J.A. (2001) Overproduction, purification and
characterization of b-1,3-glucanase type II in Escherichia
coli. Protein Express. and Purif., 23,
219-225.
130.
Pinto, J.M., Montagna,
J.M., Vecchietti, A.R., Iribarren, O.A. and Asenjo, J.A. (2001) Process
performance models in the optimization of multiproduct protein production
plants. Biotechnol. Bioeng., 74,
451-465.
129.
Graber, T.A., Taboada,
M.E., Andrews, B.A. and Asenjo, J.A. (2001) Influence of molecular weight of
the polymer in the liquid-liquid equilibrium of the poly(ethylene glycol) +
NaNO3 + H20 system at 298.15 K. J. Chem. Eng. Data., 46, 765 – 768.
128.
Gonzalez, R., Asenjo,
J.A. and Andrews, B.A. (2001) Metabolic control analysis of monoclonal antibody
synthesis. Biotechnol. Progress., 17,
217-226.
127.
Taboada, M.E., Asenjo, J.A. and Andrews, B.A. (2001) Liquid-liquid and
liquid-liquid-solid equilibria in PEG + Na2CO3 + water. Fluid Phase Equil., 108, 273-280.
126.
Andrews, A.T., Harris, D.P., Wright, G, Pyle, D.L. and Asenjo, J.A.
(2000) Affinity gel electrophoresis as a predictive technique in the
fractionation of transgenic sheep milk proteins by affinity aqueous two-phase
partitioning Biotechnol. Letts., 22, 1349–1353.
125.
Lienqueo, M.E. and
Asenjo, J.A. (2000) Use of expert systems for the synthesis of downstream
protein processes. Comput. Chem. Engng., 24,
2339-2350.
124.
Asenjo, J.A., Montagna,
J.M., Vecchietti, A.R., Iribarren, O.A. and Pinto, J.M. (2000) Strategies for
the simultaneous optimization of the structure and the process variables of a
protein production plant. Comput. Chem. Engng., 24, 2277-2290.
123.
Taboada, M.E., Graber,
T.A., Asenjo, J.A. and Andrews, B.A. (2000) Drowning-out crystallisation of
sodium sulphate using aqueous two-phase systems. J. Chromatography B, 743, 101-105.
122.
Graber, T.A., Andrews,
B.A. and Asenjo, J.A. (2000) A model for the partition of metal ions in aqueous
two-phase systems. J. Chromatography B, 743,
57-64.
121.
Shene, C., Mir, N.,
Andrews, B.A. and Asenjo (2000) Effect of the growth conditions on the
synthesis of a recombinant b-1,4-endoglucanase in continuous and fed-batch culture. Enz. Microb.
Technol., 27, 248-253.
120.
Montagna, J.M.,
Vecchietti, A., Iribarren, O.A., Pinto J.M. and Asenjo, J.A. (2000) Optimal
design of protein production plants with time and size factor process models.
Biotechnol. Progress, 16,
228-237.
119.
Salazar, O., Molitor,
J.and Asenjo, J.A. (1999) Expression and cloning of an Oerskovia xanthineolyticab–1,3-glucanase in Escherichia coli.
Biotech. Letts., 21, 797-802.
118.
Lienqueo, M.E., Salgado,
J.C. and Asenjo, J.A. (1999) An Expert System for selection of protein
purification processes: experimental validation. J. Chem. Technol. Biotechnol., 74, 293-299.
117.
Huenupi,E., Gomez, A.,
Andrews, B.A. and Asenjo, J.A. (1999) Optimization and design considerations of
two-phase continuous protein separation. J. Chem.Technol. Biotechnol., 74, 256-263.
116.
Shene, C., Andrews, B.A.
and Asenjo, J.A. (1999) Fed batch fermentations of Bacillus subtilis ToC46 (pPFF1) for the synthesis of a recombinant
β-1,3- glucanase: Experimental study and modelling. Enz. Microb. Technol., 24, 247-254.
115.
Salamanca, M.H., Merchuk,
J.C., Andrews, B.A. and Asenjo, J.A. (1998) On the kinetics of phase separation
in aqueous two-phase systems. J. Chromatography, 711, 319.
114.
Merchuk, J.C., Andrews,
B.A. and Asenjo, J.A. (1998) Aqueous two-phase systems for protein separation:
studies on phase inversion. J. Chromatography, 711, 285-293.
113.
Ferrer, P., Diers, I.,
Asenjo, J.A. and Andrews, B.A. (1998) Yeast cell permeabilizing
-1,3-glucanases: a tool for the integration of downstream processes and
metabolic engineering applications to yeast. Biotechnol. Bioeng., 58, 321-324.
112.
Rodrigues, M.I.,
Maciel-Filho, R., Asenjo, J.A., Zaror, C.A. and Maugeri, F. (1997) A procedure
for feasible and optimal operational strategies for control of CARE systems. .
J. Chem.Technol. Biotechnol. , 69,
254-260.
111.
Harris, D.P., Andrews,
A.T., Wright, G., Pyle, D.L. and Asenjo, J.A. (1997) The application of aqueous
two-phase systems to the purification of pharmaceutical proteins from
transgenic sheep milk. Bioseparation, 7,
31-37.
110.
Andrews, B.A., Nielsen,
S. and Asenjo, J.A. (1996) Partitioning and purification of monoclonal
antibodies in aqueous two-phase systems. Bioseparation, 6, 303-313.
109.
Vazquez-Lima, F., Pyle,
D.L. and Asenjo, J.A. (1997) Reaction kinetics of the esterification of lauric
acid in iso-octane using an immobilized biocatalyst. Appl. Biochem.
Biotechnol., 61, 411-422.
108.
Hachem, F., Andrews, B.A.
and Asenjo, J.A. (1996) Hydrophobic partitioning of proteins in aqueous
two-phase systems. Enzyme Microb. Technol., 19, 507-517.
107.
Andrews, B.A. and Asenjo,
J.A. (1996) Protein partitioning equilibrium between the aqueous PEG and salt
phases and the solid protein phase in PEG/salt two-phase systems. J.
Chromatography B, 685, 15-20.
106.
Parrado, J., Escuredo,
P.R., Conejero-Lara, F. Kotik, M., Ponting, C.P., Asenjo, J.A. and Dobson, C.M.
(1996) Molecular characterisation of a thermoactive β-1,3-glucanase
from Oerskovia
xanthineolytica. Biochim. Biophys. Acta, 1296, 145-151.
105.
Ferrer, P., Halkier, T.,
Hedegaard, L., Savva, D., Diers. I. and Asenjo, J.A. (1996) Nucleotide sequence
of a β-1,3-glucanase isoenzyme IIA gene of Oerskovia xanthineolytica LL G109 (Cellulomonas cellulans) and initial characterization of the
recombinant enzyme expressed in Bacillus
subtilis. J. Bacteriology., 178,
4751-4757.
104.
Asenjo, J.A. and
Chaudhuri, J.B. (1996) Innovative Separation Methods in Bioprocessing, in
Separation Processes in the Food and Biotechnology Industries: Principles and
Applications. Ed.: A.S. Grandison and M.J. Lewis, Woodhead Publ. Limited,
Cambridge, U.K., p. 179-206.
103.
Mistry, S.L., Kaul, A.,
Merchuk, J.C. and Asenjo, J.A. (1996) Mathematical Modelling and Computer
Simulation of Aqueous Two-Phase Continuous Protein Extraction. J.
Chromatography A, 741, 151-163.
102.
Asenjo, J.A., Sun, W-H.
and Spencer, J.L. (1996) Optimal control of batch processes involving
simultaneous enzymatic and microbial reactions. Bioproc. Engin., 14, 323-329.
101.
Schmidt, A.S., Andrews,
B.A. and Asenjo, J.A. (1996) Correlations for the partition behaviour of
proteins in aqueous two-phase systems. Effect of overall protein concentration.
Biotechnol. Bioeng., 50, 617-626.
100.
Lienqueo, M.E., Leser,
E.W. and Asenjo, J.A. (1996) An Expert System for the Selection and Synthesis
of Multistep Protein Separation Processes. Comput. Chem. Engng., 20, S189-S194.
99.
Lye, G.J, Asenjo, J.A. and Pyle, D.L. (1996) Reverse micellar
mass-transfer processes: spray column extraction of lysozyme. AIChE Journal, 42, 713-726.
98.
Shene, C., Mir, N., Andrews, B.A. and Asenjo, J.A. (1996) Mathematical
modelling of the synthesis of a cloned lytic β-1,3-Endoglucanase in Bacillus subtilis. Ann. N.Y. Acad. Sci., 782, 334-349.
97.
Leser, E.W., Lienqueo, M.E. and Asenjo, J.A. (1996) Implementation in an
Expert System of selection rationale for purification processes for recombinant
proteins. Ann. N.Y. Acad. Sci., 782,
441-455.
96.
Ferrer, P., Hedegaard, L., Halkier, T., Diers, I., Savva, D. and Asenjo,
J.A. (1996) Molecular cloning of a lytic β-1,3-glucanase gene from Oerskovia xanthineolytica LLG109. Ann.
N.Y. Acad. Sci., 782, 556-566 .
95.
Regalado, C., Asenjo, J.A. and Pyle, D.L. (1996) Studies on the
purification of peroxidase from horseradish roots using reverse micelles.
Enzyme Microb. Technol., 18,
332-339.
94.
Leser, E.W. and Asenjo, J.A. (1996) Process Integration in
Biotechnology, in Downstream Processing of Natural Products: A Practical
Handbook. Ed.: M.S. Verrall, John Wiley, New York., p. 123-138.
93.
Franco, T.T., Andrews, A.T. and Asenjo, J.A. (1996) Use of chemically
modified proteins to study the
effect of a single protein property on partitioning in aqueous two-phase
systems: effect of surface charge. Biotechnol. Bioeng., 49, 309-315.
92.
Franco, T.T., Andrews, A.T. and Asenjo, J.A. (1996) Use of chemically
modified proteins to study the
effect of a single protein property on partitioning in aqueous two-phase
systems: effect of surface hydrophobicity. Biotechnol. Bioeng., 49, 300-308.
91.
Franco, T.T., Andrews, A.T. and Asenjo, J.A. (1996) Conservative
chemical modification of proteins to study the effects of a single protein
property on partitioning in aqueous two-phase systems. Biotechnol. Bioeng., 49, 290-299.
90.
Kaul, A., Pereira, R.A.M., Asenjo, J.A. and Merchuk, J.C. (1995)
Kinetics of phase separation for polyethylene glycol-phosphate two-phase
systems. Biotechnol. Bioeng., 48,
246-256.
89.
Lye, G.J., Asenjo, J.A. and Pyle, D.L. (1995). Extraction of lysozyme
and ribonuclease-a using reverse micelles: limits to protein solubilization.
Biotechnol. Bioeng., 47, 509-519.
88.
Andrews, B.A., Huang, R.-B. and Asenjo, J.A. (1995). Purification of
virus like particles from yeast cells using aqueous two-phase systems.
Bioseparation, 5, 105-112.
87.
Asenjo, J.A., Schmidt, A.S., Andersen, P.R. and Andrews, B.A. (1995).
Effect of single nutrient limitation on poly-ß-hydroxybutyrate (PHB) molecular
weight distribution in Alcaligenes
eutrophus. Biotechnol. Bioeng., 46,
497-502.
86.
Vazquez-Lima, F., Pyle, D.L. and Asenjo, J.A. (1995) Factors affecting
the esterification of lauric acid using an immobilized biocatalyst: enzyme
characterization and studies in a well-mixed reactor. Biotechnol. Bioeng., 46, 69-79.
85.
Merchuk, J.C. and Asenjo, J.A. (1995) The Monod equation and mass
transfer. Biotechnol. Bioeng., 45,
91-94.
84.
Merchuk, J.C. and Asenjo, J.A. (1995). Fundamentals of Bioreactor
Design. in: Bioreactor System Design. Eds. J.A. Asenjo and J.C. Merchuk. Marcel
Dekker, New York, p. 139-206.
83.
Merchuk, J.C. and Asenjo, J.A. (1995). Design of a Bioreactor System:
Overwiew. in: Bioreactor System Design. Eds. J.A. Asenjo and J.C. Merchuk.
Marcel Dekker, New York, p. 1-12.
82.
Kaul, A. and Asenjo, J.A. (1994). Partition of Soluble Proteins from E. coli in Polyethylene Glycol-salt
Two-phase Systems, in: Separations for Biotechnology 3, Ed. D.L. Pyle, Roy.
Soc. Chem., Cambridge, p. 235-341.
81.
Ferrer, P., Mir, N., Shene, C., Halkier, T., Diers, I., Savva, D. and
Asenjo, J.A. (1994). An Integrated Approach for the Cloning, Over expression
and Use of Lytic Glucanases for the Selective Protein Recovery from Yeast
Cells, in: Separations for Biotechnology 3, Ed.D.L. Pyle, Roy. Soc. Chem.,
Cambridge, p. 159-165.
80.
Regalado, C., Asenjo, J.A., Pyle, D.L., Trinca, L.A. and Gilmour, S.
(1994) Protein extraction by reverse micelles. horseradish peroxidase recovery
maximization by surface response methodology, and denaturation studies.
Trans.Inst. Chem. Eng.,72 (C),
123-134.
79.
Turner, R.E., Baines,B.S. and Asenjo, J.A. (1994). Physico-chemical
Database Development for Baculovirus-produced Proteins: The Rational Design of
Large Scale Protein Purification, in: Separations for Biotechnology 3, Ed.D.L.
Pyle, Roy. Soc. Chem., Cambridge, p. 518-524.
78.
Regalado, C., Asenjo, J.A. and Pyle, D.L. (1994) Protein extraction by
reverse micelles. Studies on the recovery of horseradish peroxidase.
Biotechnol. Bioeng., 44, 674-681.
77.
Schmidt, A.S. and Asenjo, J.A. (1994). Modelling the Partition Behaviour
of Proteins in Aqueous Two-phase
Systems, in: Separations for Biotechnology 3, Ed. D.L. Pyle, Roy. Soc. Chem.,
Cambridge, p. 462-468.
76.
Leser, E.W. and Asenjo, J.A. (1994) Protein recovery, separation and
purification. Selection of optimal techniques using an expert system. Mem.
Inst. Oswaldo Cruz., 89, 99-109.
75.
Andrews, A.T., Noble, I. Keeratipibul, S., and Asenjo, J.A. (1994) Physicochemical properties of
the main matrix proteins of three important culture vehicles. Biotechnol.
Bioeng., 44, 29-37.
74.
Mistry, S.L., Merchuk, J.C. and Asenjo, J.A. (1994). Mathematical
Modelling and Computer Simulation of
Continuous Aqueous Two-phase Extraction,in: Separations for Biotechnology 3, Ed. D.L. Pyle, Roy. Soc. Chem.,
Cambridge, p. 321-328.
73.
Asenjo, J.A., Leser, E. and Andrews, B.A. (1994). New Perspectives in
Bioseparations, in: Separation Technology: The Next Ten Years, Ed. J. Garside,
Inst. Chem. Eng., Rugby, U.K., p. 97-131.
72.
Lye, G.J., Asenjo, J.A. and Pyle, D.L. (1994). Protein extraction using
reverse micelles: kinetics of protein partitioning. Chem. Eng. Sci., 49, 3195-3204.
71.
Asenjo, J.A., Turner, R.E., Mistry, S.L. and Kaul, A. (1994). Separation
and purification of recombinant proteins from E. coli using aqueous two-phase systems. J. of Chromatography, 668, 129-137.
70.
Asenjo, J.A., Schmidt, A.S., Hachem, F. and Andrews, B.A. (1994). Model
for predicting the partition behaviour of proteins in aqueous two-phase
systems. J. of Chromatography, 668,
47-54.
69.
Watanabe, E., Tsoka, S. and Asenjo, J.A. (1994) Selection of
chromatographic protein purification operations based on physicochemical
properties. Ann. N.Y. Acad. Sci., 721,
348-364.
68.
Andrews, B.A., Pyle, D.L. and
Asenjo, J.A. (1994) The effects of pH and ionic strength on the partitioning of
four proteins in reverse micelle systems. Biotechnol. Bioeng., 43, 1052-1058.
67.
Leser, E.W. and Asenjo, J.A. (1994). The rational selection of
purification processes for proteins: an expert system for downstream processing
design. Ann. N.Y. Acad. Sci., 721,
337-347.
66.
Schmidt, A.S., Ventom, A.M. and Asenjo, J.A. (1994). Partitioning and
purification of α-amylase in aqueous two-phase systems. Enzyme Microb.
Technol., 16, 131-142.
65.
Mistry, S.L., Asenjo, J.A. and Zaror, C.A. (1993) Mathematical modelling
and simulation of aqueous two-phase continuous protein extraction. Bioseparation, 3, 343-358.
64.
Asenjo, J.A. (1993) Cell Disintegration. in: Recovery of Bioproducts,
Ed. G. Schmidt-Kastner, SCI publications, London, p.26-35.
63.
Asenjo, J.A., Ventom, A.M., Huang, R.B. and Andrews, B.A. (1993)
Selective release of recombinant protein particles (VLPs) from yeast using a
pure lytic glucanase enzyme. Bio/Technology. 11, 214-217.
62.
Leser, E.W. and Asenjo, J.A. (1992). The rational design of purification
processes for recombinant proteins. J. of Chromatography, 584, 35-42.
61.
Asenjo, J.A. (1992). Separation Processes in Biotechnology. in:
"Profiles on Biotechnology", Eds. T.G. Villa and J. Abalde.
Publications of the Univ.of Santiago de Compostela, Spain, p.505-522.
60.
Torner, M.J., Andrews, B.A. and Asenjo, J.A. (1992). Kinetics of protein
release from yeast using enzymatic lysis for selective product recovery.
Biotechnol. Techniques., 6, 371-376.
59.
Rodriques, I., Zaror, C.A., Maugeri, F. and Asenjo, J.A. (1992)
Dynamic modelling, simulation and
control of continuous adsorption recycle extraction. Chem. Eng. Sci., 47, 263-269.
58.
Asenjo, J.A. and Maugeri, F. (1992) An Expert System for Selection and
Synthesis of Protein Purification Processes. in: "Frontiers in
Bioprocessing II", Eds. P. Todd, S. Sikdar, and M. Bier, ACS books
Washington, p. 358-379.
57.
Andrews, B.A., Torner, M.J. and Asenjo, J.A. (1991). Differential
Product Release from Yeast Cells by Selective Enzymatic Lysis. in: Upstream and
Downstream Processing in Biotechnology III, Eds. A. Huyghebaert and E.Vandamme,
Royal Flemish Society of Engineers, Antwerpen. p.3.1-3.12.
56.
Asenjo, J.A. (1991). The Rational Design of Large Scale Protein
Separation Sequences in: Upstream and Downstream Processing in Biotechnology
III, Eds. A. Huyghebaert and E. Vandamme, Royal Flemish Society of Engineers,
Antwerpen. p. 4.7-4.17.
55.
Torner, M.J and Asenjo, J.A. (1991) Kinetics of enzyme release from
breadmaking yeast cells in a bead mill. Biotechnol. Techniques. 5,
101-106.
54.
Asenjo, J.A., Parrado, J. and Andrews, B.A. (1991) The rational design
of purification processes for recombinant proteins. in Ann. N.Y. Acad. Sci., 646, 334-356.
53.
Huang, R.B., Andrews, B.A. and Asenjo, J.A. (1991) Differential product
release (DPR) of proteins from yeast: a new technique for selective product
recovery. Biotechnol. Bioeng. 38, 977-985.
52.
Asenjo, J.A., Sun, W.H. and Spencer, J.L. (1991) Optimization of batch
processes involving simultaneous enzymatic and microbial reactions. Biotechnol. Bioeng. 37, 1087-1094.
51.
Cascone, O., Andrews, B.A. and Asenjo, J.A. (1991) Partition and purification
of thaumatin in aqueous two-phase systems. Enzyme Microb. Technol. 13, 629-635.
50.
Asenjo, J.A., Franco, T., Andrews, A.T. and Andrews, B.A. (1991)
Affinity Separation of Proteins in Aqueous Two-Phase Systems, in Biologicals
from Recombinant Microorganisms and Animal Cells - New Strategies in Production
and Recovery, Eds. M. White, S. Reuveny and A. Shaffermann, VCH Publishers,
Germany, p. 439-454.
49.
Andrews, B.A., Huang, R. and Asenjo, J.A. (1991) Differential Product Release from Yeast Cells
by Selective Enzymatic Lysis, in Biologicals from Recombinant Microorganisms
and Animal Cells - New Strategies in Production and Recovery, Eds. M. White, S.
Reuveny and A. Shaffermann, VCH Publishers, Germany, p.307-321.
48.
Ventom, A.M. and Asenjo, J.A. (1991) Characterisation of yeast lytic
enzymes from Oerskovia xanthineolytica LL-G109. Enzyme Microb. Technol. 13, 71-75.
47.
Asenjo, J.A. (1990) Cell Disruption and Removal of Insolubles, in
Separations for Biotechnology II, Ed. D.L. Pyle, Elsevier, p. 11-20.
46.
Andrews, B.A., Huang, R. and Asenjo, J.A. (1990) Differential Product
Release from Yeast Cells By Selective Enzymatic Lysis, in Separations for
Biotechnology II, Ed. D.L. Pyle, Elsevier. p. 21-28.
45.
Asenjo, J.A. (1990) The Rational Design of Large Scale Protein
Separation Process, in Separations for Biotechnology II, Ed. D.L. Pyle,
Elsevier. p.. 519-528.
44.
Franco, T., Andrews, B.A., Cascone, O., Hodgson, C., Andrews, A.T. and
Asenjo, J.A. (1990) Affinity Separation of Proteins in Aqueous Two-Phase
Systems, in Separations for Biotechnology II, Ed. D.L. Pyle, Elsevier. p.
335-344.
43.
Ventom, A.M. and Asenjo, J.A. (1990) Two extracellular proteases from Oerskovia xanthineolytica LL-G109.
Biotechnol. Techniques, 4, 171-176.
42.
Ventom, A.M. and Asenjo, J.A.. (1990) Purification of the major
glucanase of Oerskovia xanthineolytica LL-G109. Biotechnol. Techniques, 4,
165-170.
41.
Andrews, B.A., Head, D.M., Dunthorne, P and Asenjo, J.A. (1990) PEG
activation and ligand binding for the affinity partitioning of proteins in
aqueous two-phase systems. Biotechnol.
Techniques, 4, 49-54.
40.
Asenjo, J.A. (1990) Selection of Operations in Separation Processes in "Separation Processes in Biotechnology" Ed. J.A. Asenjo,
Marcel Dekker, New York, p.3-16.
39.
Hunter, J.B. and Asenjo, J.A. (1990) A population balance model of enzymatic lysis of microbial cells. Biotechnol. Bioeng. 35,31-42.
38.
Prokopakis, G.J. and Asenjo, (1990) J.A. Synthesis of Downstream
Processes, in "Separation Processes in Biotechnology" Ed. J.A.
Asenjo, Marcel Dekker, New York, p.571-601.
37.
Asenjo, J.A. and Andrews, B.A. (1990) Enzymatic Cell Lysis for Product
Release in "Separation Processes in Biotechnology" Ed. J.A. Asenjo,
Marcel; Dekker, New York, p.143-175.
36.
Asenjo, J.A. and Patrick, I. (1990) Large Scale Protein Purification,
Chapter
35.
Andrews, B.A. and Asenjo, J.A. (1989) Aqueous two phase partitioning in
"Protein Purification Methods: a Practical Approach" Eds. E.L.V.
Harris and S. Angal, IRL press, p. 161-174.
34.
Asenjo, J.A., Herrera, L. and Byrne, B. (1989) Development of an Expert
System for selection and synthesis of protein purification processes. J. Biotechnol., 11, 275-298.
33.
Head, D.M. Andrews, B.A. and Asenjo, J.A. (1989) Epoxy oxyrane
activation of PEG for protein ligand coupling. Biotech. Techniques, 3, 27-32.
32.
Asenjo, J. A. and Andrews, B.A. (1989) Design and use of enzyme systems
for selective product release from microbial cells. Bioproducts and
Bioprocesses. Eds. A. Fiechter et al., Spinger Verlag, 223-234.
31.
Asenjo, J.A. Andrews, B.A. and Pitts, (1988) J.M. Design of enzyme
systems for selective product release from microbial cells: isolation of a
recombinant protein from yeast. Ann. N.Y. Acad. Sci. 542, 140-152.
30.
Liu, L.C., Prokopakis, G.J. and
Asenjo, J.A. (1988) Optimization of enzymatic lysis of yeast. Biotechnol.
Bioeng., 32, 1113-1127.
29.
Hunter, J. B. and Asenjo, J.A. (1988) A structured mechanistic model of
the kinetics of enzymatic lysis and
disruption of yeast cells. Biotechnol. Bioeng. 31, 929-944.
28.
Hunter, J.B. and Asenjo, J.A. (1987) Distributed model of enzymatic
lysis and disruption of yeast cells. Ann. N.Y. Acad. Sci., 506,
649-656.
27.
Andrews, B.A. and Asenjo, J.A. (1987) Production of enzyme systems
in continuous culture for the
controlled lysis of microbial cells. Ann. N.Y. Acad. Sci. 506, 637-641.
26.
Andrews, B.A. and Asenjo, J.A. (1987) Enzymatic lysis and disruption of
microbial cells. Trends in Biotechnol. 5, 273-277.
25.
Andrews, B.A., and Asenjo, J.A. (1987) Continuous culture studies of the
synthesis and regulation of extracellular (1-3) glucanase and protease
enzymes from Oerskovia xanthineolytica.
Biotechnol. Bioeng. 30, 628-637.
24.
Hunter, J.B. and Asenjo, J.A. (1987) Kinetics of enzymatic lysis and disruption of yeast
cells:
23.
Hunter, J.B. and Asenjo, J.A. (1987) Kinetics of enzymatic lysis and
disruption of yeast cells: 1. Evaluation of two lytic systems with different
properties. Biotechnol. Bioeng. 30, 471-480.
22.
Asenjo, J.A. and Suk, J., (1986) Microbial conversion of methane into
poly-ß hydroxybutyrate (PHB): growth and intracellular product accumulation in
a type II methanotroph. J. Ferment. Technol. 64, 271-278.
21.
Hunter, J.B. and Asenjo, J.A., (1986) Structured and simple models of
enzymatic lysis and disruption of yeast cells, in "Separations, Recovery
and Purification in Biotechnology; Recent Advances and Mathematical
Modeling", Eds. J.A. Asenjo and J. Hong, ACS books, Vol 314, Washington, 9-31.
20.
Andrews, B.A. and Asenjo, J.A., (1986) Synthesis and regulation of
extracellular β(1-3) glucanase and protease by Cytophaga sp. in batch and continuous culture. Biotechnol. Bioeng. 28, 1366-1375.
19.
Asenjo, J.A., Spencer, J.L. and Phuvan, V. (1986) Optimization of the
simultaneous saccharification and fermentation of an insoluble substrate into
microbial metabolites. Ann. N.Y. Acad.
Sci. 469, 404-420.
18.
Enzminger, J.D. and Asenjo, J.A. (1986) Use of cell recycle in the
aerobic fermentative production of citric acid by yeast. Biotech. Lett., 8, 7-12.
17.
Asenjo, J.A. and Suk, J. (1985) Kinetics and models of the bioconversion
of Methane into an intracellular polymer, Poly-ß-hydroxybutyrate (PHB),
Biotechnol. Bioeng. S15, 225-234.
16.
Asenjo, J.A., Andrews, B.A., Hunter, J.B. and LeCorre, S. (1985)
Microbial cell-lytic enzyme systems: production and reaction kinetics. Process Biochem. 20, 158-164.
15.
Asenjo, J.A., Amar, M., Cartagena, N., King, J., Hiche, E., and Stekel,
A. (1985) Use of a heme-iron concentrate in the fortification of biscuits. J.
Food Sci., 50, 794-798.
14.
LeCorre, S., Andrews, B.A. and Asenjo, J.A. (1985) Use of a lytic enzyme
system from Cytophaga sp. in the
lysis of Gram- positive bacteria. Enzyme
Microb. Technol. 7, 73-78.
13.
Asenjo, J.A. (1984) Modeling the bioconversion of cellulose into
microbial products: rate limitations. Process Biochem. 19, 217-224.
12.
Asenjo, J.A. (1983) Process kinetics and bioreactor design for the
direct bioconversion of cellulose into microbial products. Biotechnol. Bioeng., S13, 449-456.
11.
Asenjo, J.A. (1983) Maximizing the formation of glucose in the enzymatic
hydrolysis of insoluble cellulose. Biotechnol. Bioeng., 25, 3185-3190.
10.
Asenjo, J.A. and Jew, C. (1983) Primary metabolite or microbial protein
from cellulose. Conditions, kinetics and
modelling of the simultaneous saccharification and fermentation of citric acid.
Ann. N.Y. Acad. Sci., 413, 211-217.
9.
Asenjo, J.A., Szuhay, J.,
and Chiu, D. (1982) Growth and citric acid production by Candida guilliermondii using a cellulose substrate. Biotechnol.
Bioeng., S12, 111-120.
8.
Hernandez, R. and Asenjo,
J.A. (1982) Production and characterization of an enzymatic hydrolysate of skim
milk lactose and proteins. J. Food Sci., 47,
1895 -1898 and 1911.
7.
Contreras, I., Gonzalez, R., Ronco, A., and Asenjo, J.A. (1982). Cellulolytic enzymes for the hydrolysis of leached beet cosette.
Biotech. Lett., 4, 51-56.
6.
Asenjo, J.A. (1981)
Process for the production of yeast-lytic enzymes and the disruption of whole
yeast cells. In: "Advances in
Biotechnology,", Vol. 3 (C. Vezina and K. Singh, eds.). p. 295-300, Pergamon Press.
5.
Asenjo, J.A. and Dunnill,
P. (1981) The isolation of lytic enzymes from Cytophaga sp. and their application to the rupture of yeast cells.
Biotechnol. Bioeng., 23, 1045-1056.
4.
Asenjo, J.A., Dunnill,
P., and Lilly, M.D. (1981) The
production of yeast-lytic enzymes by Cytophaga in batch culture. Biotechnol. Bioeng., 23, 97-109.
3.
Asenjo, J.A. (1980)
Continuous isolation of yeast-lytic enzymes. In:"Enzyme Engineering,"
Vol. 5 (H. Weetall and G. Royer, eds.), p. 49-55, Plenum Press, New York.
2.
Halling, P.J., Asenjo,
J.A., and Dunnill, P. (1979) Non-porous magnetic supports for proteases,
cell-lytic enzymes and ribonuclease: limits of reactant size. Biotechnol.
Bioeng., 21, 2359-2363.
1.
Asenjo, J.A., Munoz R.,
and Pyle, D.L. (1977) On the transition from a fixed to a spouted bed. Chem. Eng. Sci., 32, 109-117.
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